Paper: Use of gene expression profiling to direct molecular imaging of lung cancer
Authors:
Jan Grimm, David G. Kirsch, Stephen D. Windsor, Carla F. Bender Kim, Philip M. Santiago, Vasilis Ntziachristos, Center for Molecular Imaging Research and Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, David G.
712 kb, 14404--14409 PNAS October 4, 2005 vol. 102 no. 40, Edited by George Klein, Karolinska Institutet, Stockholm, Sweden,
Abstract:
Quoted authors' abstract:
"Using gene expression profiling, we identified cathepsin cysteine proteases as highly up--regulated genes in a mouse model of human lung adenocarcinoma. Overexpression of cathepsin proteases
in these lung tumors was confirmed by immunohistochemistry
and Western blotting. Therefore, an optical probe activated
by cathepsin proteases was selected to detect murine lung tumors
in vivo as small as 1 mm in diameter and spatially separated. We
generated 3D maps of the fluorescence signal and fused them with
anatomical computed tomography images to show a close correlation
between fluorescence signal and tumor burden. By serially
imaging the same mouse, optical imaging was used to follow
tumor progression. This study demonstrates the capability for
molecular imaging of a primary lung tumor by using endogenous
proteases expressed by a tumor. It also highlights the feasibility of
using gene expression profiling to identify molecular targets for
imaging lung cancer."
Rights:
Open access
http://www.pnas.org cgi_doi10.1073_pnas.0503920102
